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mc sv huc t 2  (ATCC)


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    Structured Review

    ATCC mc sv huc t 2
    Mc Sv Huc T 2, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mc+sv+huc+t+2/MC-SV-HUC+T-2/pm41286047-76-21-27
    Average 90 stars, based on 12 article reviews
    mc sv huc t 2 - by Bioz Stars, 2026-09
    90/100 stars

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    Cell Culture:

    Article Title: Tumor-derived lactate fuels the STAT3-LCN2 pathway to promote bladder cancer malignancy and chemoresistance.
    Article Snippet: .. Cell culture, transfection and infection Human bladder cancer cell lines 5637 (obtained from the Bioresource Collection and Research Center, Taiwan) and MC-SV-HUC T-2 (MC-T2; obtained from the American Type Culture Collection, USA) were cultured in RPMI-1640 medium (Corning, Cat#50-020-PC, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Cat#10437028, Waltham, USA) and 100 μg/ml streptomycin and 100 U/ml penicillin (Corning, Cat. 30-002-CI, NY, USA). ..

    Article Title: Gain of TPPP as a predictor of progression in patients with bladder cancer
    Article Snippet: The cells were cultured in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 units/ml penicillin and 100 μg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.), and 2 mM L-glutamine (Gibco; Thermo Fisher Scientific, Inc.). .. SV-HUC-1 cells, a normal human uroepithelial cell line and MC-SV-HUC T-2, a tumorigenic human urothelial cell line, were purchased from the American Type Culture Collection and cultured in Ham's F12 medium (Gibco; Thermo Fisher Scientific, Inc.) containing 7% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin and 2 mM L-glutamine. ..

    Article Title: Tumor-derived lactate fuels the STAT3-LCN2 pathway to promote bladder cancer malignancy and chemoresistance
    Article Snippet: .. Human bladder cancer cell lines 5637 (obtained from the Bioresource Collection and Research Center, Taiwan) and MC-SV-HUC T-2 (MC-T2; obtained from the American Type Culture Collection, USA) were cultured in RPMI-1640 medium (Corning, Cat#50-020-PC, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Cat#10437028, Waltham, USA) and 100 μg/ml streptomycin and 100 U/ml penicillin (Corning, Cat. 30-002-CI, NY, USA). ..

    Article Title: Gain of TPPP as a predictor of progression in patients with bladder cancer.
    Article Snippet: The cells were cultured in RPMI 1640 medium (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 units/ml penicillin and 100 μg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.), and 2 mM L‐glutamine (Gibco; Thermo Fisher Scientific, Inc.). .. SV‐HUC‐1 cells, a normal human uroepithelial cell line and MC‐SV‐HUC T‐2, a tumorigenic human urothelial cell line, were purchased from the American Type Culture Collection and cultured in Ham's F12 medium (Gibco; Thermo Fisher Scientific, Inc.) containing 7% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin and 2 mM L‐glutamine. ..

    Transfection:

    Article Title: Tumor-derived lactate fuels the STAT3-LCN2 pathway to promote bladder cancer malignancy and chemoresistance.
    Article Snippet: .. Cell culture, transfection and infection Human bladder cancer cell lines 5637 (obtained from the Bioresource Collection and Research Center, Taiwan) and MC-SV-HUC T-2 (MC-T2; obtained from the American Type Culture Collection, USA) were cultured in RPMI-1640 medium (Corning, Cat#50-020-PC, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Cat#10437028, Waltham, USA) and 100 μg/ml streptomycin and 100 U/ml penicillin (Corning, Cat. 30-002-CI, NY, USA). ..

    Infection:

    Article Title: Tumor-derived lactate fuels the STAT3-LCN2 pathway to promote bladder cancer malignancy and chemoresistance.
    Article Snippet: .. Cell culture, transfection and infection Human bladder cancer cell lines 5637 (obtained from the Bioresource Collection and Research Center, Taiwan) and MC-SV-HUC T-2 (MC-T2; obtained from the American Type Culture Collection, USA) were cultured in RPMI-1640 medium (Corning, Cat#50-020-PC, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Cat#10437028, Waltham, USA) and 100 μg/ml streptomycin and 100 U/ml penicillin (Corning, Cat. 30-002-CI, NY, USA). ..

    other:

    Article Title: Cross-Contamination of a UROtsa Stock with T24 Cells – Molecular Comparison of Different Cell Lines and Stocks
    Article Snippet: Available ureter-derived cell lines are 639-V, Hs 789.T, MC-SV-HUC T-2, and SV-HUC-1 ( www.cellbankaustralia.com , www.dmsz.de , cellbank.nibio.go.jp, www.atcc.org ).



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    ATCC mc sv huc t2 cells
    Effect of SOX2 on the proliferation and stemness of UTUC cells. ( A and B ): SOX2-specific shRNAs significantly decreased the expression level of SOX2 in UM-UC-14 and MC-SV-HUC <t>T2</t> cells. ( C and D ): Cell proliferation changes in UTUC cells were determined using an EdU assay. ( E and F ): Cell proliferation changes in UTUC cells were determined using a colony-formation assay. ( G and H ): The cell stemness of UTUC cells was determined using a sphere-forming assay. Cell proliferation was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. Data are shown as the mean ± SD. **p < 0.01.
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    Image Search Results


    Effect of SOX2 on the proliferation and stemness of UTUC cells. ( A and B ): SOX2-specific shRNAs significantly decreased the expression level of SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. ( C and D ): Cell proliferation changes in UTUC cells were determined using an EdU assay. ( E and F ): Cell proliferation changes in UTUC cells were determined using a colony-formation assay. ( G and H ): The cell stemness of UTUC cells was determined using a sphere-forming assay. Cell proliferation was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. Data are shown as the mean ± SD. **p < 0.01.

    Journal: Cancer Management and Research

    Article Title: Increased Expression Of SOX2 Predicts A Poor Prognosis And Promotes Malignant Phenotypes In Upper Tract Urothelial Carcinoma

    doi: 10.2147/CMAR.S219568

    Figure Lengend Snippet: Effect of SOX2 on the proliferation and stemness of UTUC cells. ( A and B ): SOX2-specific shRNAs significantly decreased the expression level of SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. ( C and D ): Cell proliferation changes in UTUC cells were determined using an EdU assay. ( E and F ): Cell proliferation changes in UTUC cells were determined using a colony-formation assay. ( G and H ): The cell stemness of UTUC cells was determined using a sphere-forming assay. Cell proliferation was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. Data are shown as the mean ± SD. **p < 0.01.

    Article Snippet: The UM-UC-14 cell line (Sigma-Aldrich, St. Louis, MO, USA) was a generous gift from Prof. Kitramura of Toyama University, MC-SV-HUC T2 cells were purchased from ATCC (CRL-9519), and SV-HUC-1 cells were purchased from the Institute of Cell Research (Chinese Academy of Sciences, Shanghai, China).

    Techniques: Expressing, EdU Assay, Colony Assay

    Effect of SOX2 on the migration and invasion of UTUC cells. ( A and B ): The migratory abilities of UTUC cells were determined using a wound healing assay. Cell migration was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. ( C and D ): The invasive abilities of UTUC cells were determined using a transwell assay. Cell invasion was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. Data are shown as the mean ± SD. *p < 0.05; **p < 0.01.

    Journal: Cancer Management and Research

    Article Title: Increased Expression Of SOX2 Predicts A Poor Prognosis And Promotes Malignant Phenotypes In Upper Tract Urothelial Carcinoma

    doi: 10.2147/CMAR.S219568

    Figure Lengend Snippet: Effect of SOX2 on the migration and invasion of UTUC cells. ( A and B ): The migratory abilities of UTUC cells were determined using a wound healing assay. Cell migration was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. ( C and D ): The invasive abilities of UTUC cells were determined using a transwell assay. Cell invasion was inhibited by silencing SOX2 in UM-UC-14 and MC-SV-HUC T2 cells. Data are shown as the mean ± SD. *p < 0.05; **p < 0.01.

    Article Snippet: The UM-UC-14 cell line (Sigma-Aldrich, St. Louis, MO, USA) was a generous gift from Prof. Kitramura of Toyama University, MC-SV-HUC T2 cells were purchased from ATCC (CRL-9519), and SV-HUC-1 cells were purchased from the Institute of Cell Research (Chinese Academy of Sciences, Shanghai, China).

    Techniques: Migration, Wound Healing Assay, Transwell Assay